H2N2V1, 1968, Analysis, bibRecord, 000095

Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus.

Identifieur interne : 000095 ( 1968/Analysis ); précédent : 000094; suivant : 000096

Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus.

Auteurs : Michelle Roti [États-Unis] ; Junbao Yang ; Deanna Berger ; Laurie Huston ; Eddie A. James ; William W. Kwok

Source :

Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 2008.

RBID : pubmed:18209073

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, CD4-Positive T-Lymphocytes (immunology), Cross Reactions, Epitopes, T-Lymphocyte (chemistry), Epitopes, T-Lymphocyte (immunology), Female, Hemagglutinins, Viral (chemistry), Hemagglutinins, Viral (immunology), Humans, Influenza A Virus, H5N1 Subtype (immunology), Influenza, Human (immunology), Male, Molecular Sequence Data, Viral Proteins (chemistry), Viral Proteins (immunology).
MESH :
- chemical , chemistry : Epitopes, T-Lymphocyte, Hemagglutinins, Viral, Viral Proteins.
- immunology : CD4-Positive T-Lymphocytes, Epitopes, T-Lymphocyte, Hemagglutinins, Viral, Influenza A Virus, H5N1 Subtype, Influenza, Human, Viral Proteins.
- Amino Acid Sequence, Cross Reactions, Female, Humans, Male, Molecular Sequence Data.

Abstract

It is commonly perceived that the human immune system is naive to the newly emerged H5N1 virus. In contrast, most adults have been exposed to influenza A H1N1 and H3N2 viruses through vaccination or infection. Adults born before 1968 have likely been exposed to H2N2 viruses. We hypothesized that CD4(+) T cells generated in response to H1N1, H3N2, and H2N2 influenza A viruses also recognize H5N1 epitopes. Tetramer-guided epitope mapping and Ag-specific class II tetramers were used to identify H5N1-specific T cell epitopes and detect H5N1-specific T cell responses. Fifteen of 15 healthy subjects tested had robust CD4(+) T cell responses against matrix protein, nucleoprotein, and neuraminidase of the influenza A/Viet Nam/1203/2004 (H5N1) virus. These results are not surprising, because the matrix protein and nucleoprotein of influenza A viruses are conserved while the neuraminidase of the H5N1 virus is of the same subtype as that of the circulating H1N1 influenza strain. However, H5N1 hemagglutinin-reactive CD4(+) T cells were also detected in 14 of 14 subjects examined despite the fact that hemagglutinin is less conserved. Most were cross-reactive to H1, H2, or H3 hemagglutinin epitopes. H5N1-reactive T cells were also detected ex vivo, exhibited a memory phenotype, and were capable of secreting IFN-gamma, TNF-alpha, IL-5, and IL-13. These data demonstrate the presence of H5N1 cross-reactive T cells in healthy Caucasian subjects, implying that exposure to influenza A H1N1, H3N2, or H2N2 viruses through either vaccination or infection may provide partial immunity to the H5N1 virus.

DOI: 10.4049/jimmunol.180.3.1758
PubMed: 18209073

Affiliations:

Links to Exploration step

pubmed:18209073

Le document en format XML

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<author><name sortKey="Yang, Junbao" sort="Yang, Junbao" uniqKey="Yang J" first="Junbao" last="Yang">Junbao Yang</name>
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<author><name sortKey="Berger, Deanna" sort="Berger, Deanna" uniqKey="Berger D" first="Deanna" last="Berger">Deanna Berger</name>
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<author><name sortKey="Huston, Laurie" sort="Huston, Laurie" uniqKey="Huston L" first="Laurie" last="Huston">Laurie Huston</name>
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<author><name sortKey="Berger, Deanna" sort="Berger, Deanna" uniqKey="Berger D" first="Deanna" last="Berger">Deanna Berger</name>
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<author><name sortKey="Huston, Laurie" sort="Huston, Laurie" uniqKey="Huston L" first="Laurie" last="Huston">Laurie Huston</name>
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<series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>CD4-Positive T-Lymphocytes (immunology)</term>
<term>Cross Reactions</term>
<term>Epitopes, T-Lymphocyte (chemistry)</term>
<term>Epitopes, T-Lymphocyte (immunology)</term>
<term>Female</term>
<term>Hemagglutinins, Viral (chemistry)</term>
<term>Hemagglutinins, Viral (immunology)</term>
<term>Humans</term>
<term>Influenza A Virus, H5N1 Subtype (immunology)</term>
<term>Influenza, Human (immunology)</term>
<term>Male</term>
<term>Molecular Sequence Data</term>
<term>Viral Proteins (chemistry)</term>
<term>Viral Proteins (immunology)</term>
</keywords>
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<term>Déterminants antigéniques des lymphocytes T ()</term>
<term>Déterminants antigéniques des lymphocytes T (immunologie)</term>
<term>Femelle</term>
<term>Grippe humaine (immunologie)</term>
<term>Humains</term>
<term>Hémagglutinines virales ()</term>
<term>Hémagglutinines virales (immunologie)</term>
<term>Lymphocytes T CD4+ (immunologie)</term>
<term>Mâle</term>
<term>Protéines virales ()</term>
<term>Protéines virales (immunologie)</term>
<term>Réactions croisées</term>
<term>Sous-type H5N1 du virus de la grippe A (immunologie)</term>
<term>Séquence d'acides aminés</term>
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<term>Hemagglutinins, Viral</term>
<term>Viral Proteins</term>
</keywords>
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<term>Grippe humaine</term>
<term>Hémagglutinines virales</term>
<term>Lymphocytes T CD4+</term>
<term>Protéines virales</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term>
<term>Epitopes, T-Lymphocyte</term>
<term>Hemagglutinins, Viral</term>
<term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza, Human</term>
<term>Viral Proteins</term>
</keywords>
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<term>Cross Reactions</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Molecular Sequence Data</term>
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<term>Déterminants antigéniques des lymphocytes T</term>
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<term>Humains</term>
<term>Hémagglutinines virales</term>
<term>Mâle</term>
<term>Protéines virales</term>
<term>Réactions croisées</term>
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<front><div type="abstract" xml:lang="en">It is commonly perceived that the human immune system is naive to the newly emerged H5N1 virus. In contrast, most adults have been exposed to influenza A H1N1 and H3N2 viruses through vaccination or infection. Adults born before 1968 have likely been exposed to H2N2 viruses. We hypothesized that CD4(+) T cells generated in response to H1N1, H3N2, and H2N2 influenza A viruses also recognize H5N1 epitopes. Tetramer-guided epitope mapping and Ag-specific class II tetramers were used to identify H5N1-specific T cell epitopes and detect H5N1-specific T cell responses. Fifteen of 15 healthy subjects tested had robust CD4(+) T cell responses against matrix protein, nucleoprotein, and neuraminidase of the influenza A/Viet Nam/1203/2004 (H5N1) virus. These results are not surprising, because the matrix protein and nucleoprotein of influenza A viruses are conserved while the neuraminidase of the H5N1 virus is of the same subtype as that of the circulating H1N1 influenza strain. However, H5N1 hemagglutinin-reactive CD4(+) T cells were also detected in 14 of 14 subjects examined despite the fact that hemagglutinin is less conserved. Most were cross-reactive to H1, H2, or H3 hemagglutinin epitopes. H5N1-reactive T cells were also detected ex vivo, exhibited a memory phenotype, and were capable of secreting IFN-gamma, TNF-alpha, IL-5, and IL-13. These data demonstrate the presence of H5N1 cross-reactive T cells in healthy Caucasian subjects, implying that exposure to influenza A H1N1, H3N2, or H2N2 viruses through either vaccination or infection may provide partial immunity to the H5N1 virus.</div>
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<name sortKey="Kwok, William W" sort="Kwok, William W" uniqKey="Kwok W" first="William W" last="Kwok">William W. Kwok</name>
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<country name="États-Unis"><region name="Washington (État)"><name sortKey="Roti, Michelle" sort="Roti, Michelle" uniqKey="Roti M" first="Michelle" last="Roti">Michelle Roti</name>
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Serveur d'exploration H2N2

Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus.

Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
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